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产品信息
ABS 046-03-02
产品规格
种属反应
Human,
Mammal,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
03
抗原
Synthetic GLP-1(9-37) adsorbed onto aluminum hydroxide gel.
偶联物
形式
Liquid
浓度
1.03 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536223
产品详细信息
Specificity: ABS 046-03-02 binds GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37). This antibody shows 0-16% cross-Specificity with C-terminally amidated GLP-1 in various circumstances.
Reactivity: ABS 046-03-02 binds free GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37) in solution. Two different ELISA sandwich setups can be used for measuring non-amidated GLP-1 forms: The first uses ABS 046-03 as a capture antibody with biotinylated detection antibody ABS 033-10-02. This shows ~5% cross-reactivity with C-terminally amidated GLP-1. The second combination uses HYB 147-12 as a capture antibody with biotinylated detection antibody ABS 046-03-02, resulting in ~16% cross-reactivity with C-terminally amidated GLP-1. Results show detection limits of 44 pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
靶标信息
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: glicentin-related polypeptide; GLP1/2; glucagon; Glucagon like peptide 1; glucagon-like peptide 1; glucagon-like peptide 2; glucagon-like peptide-1; MGC138331; OXY; preproglucagon; Pro-glucagon
基因别名: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG
UniProt ID: (Human) P01275, (Mouse) P55095
Entrez Gene ID: (Human) 2641, (Mouse) 14526, (Rat) 24952
regulation of carbohydrate metabolic process
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
protein kinase A signaling
positive regulation of peptidyl-threonine phosphorylation
regulation of lipid metabolic process
protein metabolic process
positive regulation of cAMP biosynthetic process
positive regulation of protein binding
negative regulation of appetite
positive regulation of peptidyl-serine phosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
response to starvation
negative regulation of apoptotic process
positive regulation of protein kinase activity
regulation of insulin secretion
positive regulation of histone H3-K4 methylation
positive regulation of ERK1 and ERK2 cascade
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway
signal transduction
G-protein coupled receptor signaling pathway
feeding behavior
cell proliferation
cellular response to glucagon stimulus
